Production and application of autologous epithelial equivalents for the recovery of cleft palate in a patient
Introduction: this research paper stems from the project entitled “Production of oral mucosae equivalents for cleft lip and palate patients” undertaken by the “Tissue engineering and cell therapy” research group of the Universidad de Antioquia. One of the most frequent alterations in the oral cavity is the cleft lip and palate (cl/p) defect. Depending on the proportion and extension of the damage, conventional surgical procedures are often only partially successful. The problems are mainly due to deficiencies of donor tissue and to adjacent, concerned structures. Through tissue engineering numerous researchers have developed oral mucosae equivalents that make more tissue available for the treatment of these defects.
Materials and methods: the application of these substitutes reduces morbility in donor sites, and reestablishes the recovery and functions of affected tissue. Due to the high frequency of cl/p, we propose to develop an autologous oral mucosa substitute as a therapeutic alternative for patients with these defects. In this study, we produced the substitute from autologous tissue. We conducted studies to determine the characteristics of the equivalent for it to meet the conditions to be applied to the patient, who underwent periodic clinical monitoring to assess recovery.
Results: we observed a decrease in the defect with good cicatrization. On a histological level, we observed the presence of collagen fibers as a support matrix.
Conclusions: with this new therapeutic alternative we mean to reduce the morbidity of donor sites and increase the availability of tissue in a short time for the rehabilitation of patients
How to Cite
License
Every single author of the articles has to declare that is an original unpublished work exclusively created by them, that it has not been submitted for simultaneous evaluation by another publication and that there is no impediment of any kind for concession of the rights provided for in the contract.
In this sense, the authors committed to await the result of the evaluation by the journal Revista Nacional de Odontologia before considering its submission to another medium; in case the response by that publication is positive, additionally, the authors committed to respond for any action involving claims, plagiarism or any other kind of claim that could be made by third parties.
At the same time, the authors have to declare that they are completely in agreement with the conditions presented in their work and that they cede all patrimonial rights. These rights involve reproduction, public communication, distribution, dissemination, transformation, making it available and all forms of exploitation of the work using any medium or procedure, during the term of the legal protection of the work and in every country in the world, to the Universidad Cooperativa de Colombia Press.
Jugessur A, Murray JC. Orofacial clefting: recent insights into a complex trait. Curr Opin Genet Dev. 2005; 15: 270-8.
Stanier P, Moore GE. Genetics of cleft lip and palate: syndromic genes contribute to the incidence of nonsyndromic clefts. Hum Mol Genet. 2004; 13 Spec N.º 1: R73-R81.
Murray JC. Gene/environment causes of cleft lip and/or palate. Clin Genet. 2002; 61: 248-256.
Hillerup S, Hjorting-Hansen E, Schwartz O. Reconstruction of alveolar process in a cases of cleft lip, jaw and palate. Tandlaegebladet. 1985; 89: 511-3.
Hillerup S, Hjorting-Hansen E, Dahl E. Maxillary surgery in cleft palate reconstruction. Surgical reconstruction of teeth, mouth and jaws in cleft lip and cleft palate. Ugeskr Laeger. 1987; 149: 1521-5.
Langdon J, Williams DM, Navsaria H, Leigh IM. Autologous keratinocyte grafting: a new technique for intra-oral reconstruction. Br Dent J. 1991; 171: 87-90.
Izumi K, Feinberg SE, Iida A, Yoshizawa M. Intraoral grafting of an ex vivo produced oral mucosa equivalent: a preliminary report. Int J Oral Maxillofac Surg. 2003; 32: 188-97.
Kinikoglu B, Auxenfans C, Pierrillas P, Justin V, Breton P, Burillon C et al. construction of a fullthickness collagen-based human oral mucosal equivalent. Biomaterials. 2009; 30: 6418-25.
Izumi K, Takacs G, Terashi H, Feinberg SE. Ex vivo development of a composite human oral mucosal equivalent. J Oral Maxillofac Surg. 1999; 57: 571-7.
Lauer G, Schimming R. Tissue-engineered mucosa graft for reconstruction of the intraoral lining after freeing of the tongue: a clinical and immunohistologic study. J Oral Maxillofac Surg. 2001; 59: 169-75.
Ophof R, Maltha JC, Kuijpers-Jagtman AM, Von den Hoff JW. Implantation of tissue-engineered mucosal substitutes in the dog palate. Eur J Orthod. 2008; 30: 1-9.
Gonzalez S, Junquera L, Peña I García V, Gallego L, García E et al. In vitro culture with collagen and human fibroblasts of a full-thickness oral mucosa equivalent. Rev Esp Cir Oral y Maxilofac 2009; 31: 98-106.
Hotta T, Yokoo S, Terashi H, Komori T. Clinical and histopathological analysis of healing process of intraoral reconstruction with ex vivo produced oral mucosa quivalent. Kobe J Med Sci. 2007; 53: 1-14.
Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975; 6: 331-43.
Saddiq ZA, Barbenel JC, Grant MH. The mechanical strength of collagen gels containing glycosaminoglycans and populated with fibroblasts. J Biomed Mater
Res A. 2009; 89: 697-706.
Ho G, Barbenel J, Grant MH. Effect of low-level laser treatment of tissue-engineered skin substitutes: contraction of collagen lattices. J Biomed Opt J Biomed Opt. 2009; 14: 034002 doi: 10.1117/1.3127201.
Ueda M, Ebata K, Kaneda T. In vitro fabrication of bioartificial mucosa for reconstruction of oral mucosa: basic research and clinical application. Ann Plast Surg. 1991; 27: 540-9.
Ueda M, Hata KI, Sumi Y, Mizuno H, Niimi A. Peri-implant soft tissue management through use of cultured mucosal epithelium. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 86: 393-400.
Lauer G, Otten JE, von Specht BU, Schilli W. Cultured gingival epithelium. A possible suitable material for pre-prosthetic surgery. J Craniomaxillofac Surg. 1994; 22: 18-22.
Bodner L, Grossman N. The use of cultured mucosal graft for preprosthetic surgery. Refuat Hapeh Vehashinayim. 2001; 18: 32-4.
Raghoebar GM, Tomson AM, Scholma J, Blaauw EH, Witjes MJ, Vissink A. Use of cultured mucosal grafts to cover defects caused by vestibuloplasty: an in vivo study. J Oral Maxillofac Surg. 1995; 53: 872-8.
Lauer G. Autografting of feeder-cell free cultured gingival epithelium. Method and clinical application. J Craniomaxillofac Surg. 1994; 22: 18-22.
Bodner L, Grossman N. Autologous cultured mucosal graft to cover large intraoral mucosal defects: a clinical study. J Oral Maxillofac Surg. 2003; 61: 169-73.
Izumi K, Terashi H, Marcelo CL, Feinberg SE. Development and characterization of a tissue-engineered human oral mucosa equivalent produced in a serumfree culture system. J Dent Res. 2000; 79: 798-805.
