Producing Ethanol from Glycerin, a Biofuel by-Product, Using Saccharomyces Cerevisiae
The growing biofuel market has led to an inevitable increase in the availability of glycerin, a by-product of the production process. Due to this abundant supply of cheap unrefined glycerin, researchers are looking for new alternative uses. This research analyzes how the glycerin that comes from the production of palm oil biofuel could be used as the raw material for producing chemicals with high commercial value through fermenting agents, thus providing the basis for future bio-refineries. By mixing glycerin with different substrates via an anaerobic microbial fermentation process using a strain of Saccharomyces cerevisiae and prior separation, different products with high added value, such as ethanol, can be obtained.
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Gobierno Federal-Secretaria de Energia, Prospectiva del mercado de petroleo crudo 2010-2025. México, 2011. [En Linea]. Disponible en: http://www.sener.gob.mx
Asociación Colombiana de Petróleo, “Informe estadístico petrolero 2011”, Bogotá, 2012. [En linea]. Disponible en: http://www.acp.com.co
A. Demirbas, “Biodiesel A Realistic Fuel Alternative for Diesel Engines”, Springer London, 2008.
F. Yang, M. Hanna, y R. Sun, “Value-added uses for crude glycerol‑a byproduct of biodiesel production”. Biotechnology for Biofuels, Vol. 5, pp. 1‑10, No. 13, 2012.
M. Hasheminejad, M. Tabatabaei, Y. Mansourpanah, M.Khatami y A. Javani, “Upstream and downstream strategies to economize biodiesel production”. Bioresource Technology, Vol. 102, No. 2, pp. 461‑468, 2011.
A. Singhabhandhu y T. Tezuka, “A perspective on incorporation of glycerin purification process in biodiesel plants using waste cooking oil as feedstock”. Energy, Vol.35, pp. 2493‑2504, 2010.
M. Hajek y F. Skopal, “Treatment of glycerol phase formed by biodiesel production”. Bioresource Technology, Vol. 101, No. 9, pp. 3242‑3245, 2010.
S. Yazdani y R. Gonzalez, “Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry”. Current opinion in biotechnology. Vol. 7, No.18, pp. 213‑219, 2007.
W. Crueger y A. Crueger, Biotecnologia: Manual de Microbiologia Industrial, Zaragoza: Editorial Acribia, 1993,pp. 143-146.
K. Yu, S. Kim y S. Han, “Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae”. Bioresource Technology, Vol. 101, pp. 4157‑4161, 2010.
C. Cardona y C. Orrego, Avances investigativos en la producción de biocombustibles, Manizales: Universidad Nacional de Colombia, 2009.
N. Akhtar, A. Pahlman, K. Larsson, A. Corbett y L.Adler, “SGD1 encodes an essential nuclear protein of Saccharomyces cerevisiae that affects expression of the
GPD1 gene for glycerol 3-phosphate dehydrogenase”, febs Lett. Vol. 483, No. 2‑3, pp. 87‑92, 2000.
R. Gonzalez, A. Murarka, Y. Dharmadi, y S. Yazdani, “A new model for the anaerobic fermentation of glycerol in enteric bacteria: Trunk and auxiliary pathways in Escherichia coli”. Metabolic Engineering, Vol. 10, No. 5, pp. 234‑245, 2008.
T. Ito, Y. Nakashimada, K. Senba, T. Matsui y N. Nishio, “Hydrogen and Ethanol Production from Glycerol - Containing Wastes Discharges after Biodiesel Manufacturing Process”. Journal of Bioscience and Bioengineering, Vol. 100, No. 3, pp. 260‑265, 2005.
Y. Dharmadi, A. Murarka, R. Gonzalez y S. Yazdani, “A new model for the anaerobic fermentation of glycerol in enteric bacteria: Trunk and auxiliary pathways”. Escherichia coli. Metabolic Engineering, Vol. 10, No. 5, pp.234‑245, 2008.
C. Kasavi, I. Finore, L. Lama, B. Nicolaus, G. Oliver, E.Toksoy y B. Kirdar, “Evaluation of industrial Saccharomyces cerevisiae strains for ethanol production from biomass”.Biomass and Bioenergy, Vol. 45, pp. 230‑238, 2012.
K. Yu, S. Kim y S. Han, “Reduction of glycerol production to improve ethanol yield in an engineered Saccharomycescerevisiae using glycerol as a substrate”. Journal of Biotechnology, Vol. 150, pp. 209‑214, 2010.
A. Ochoa‑Estopier, N. Lesage, N. Gorret y S. Guillouet, “Kinetic analysis of a Saccharomyces cerevisiae strain adapted for improved growth on glycerol: Implications for the development of yeast bioprocesses on glycerol”. Bioresource Technology, Vol. 102, pp. 1521‑1527, 2011.
