Células madre mesenquimales: una alternativa para la regeneración ósea

Lina María Franco González; Luz Marina Restrepo Múnera

Mesenchymal stem cells : an alternative for bone regeneration

Review Article

Lina María Franco González Ver Biografía

Universidad Cooperativa de Colombia, sede Medellín

Luz Marina Restrepo Múnera Ver Biografía

Universidad de Antioquia

Mesenchymal Stem Cells (msc) are found in the bone marrow and have the capacity for self-renewal and differentiation in multiple lineages: osteogenic, condrogenic, adipogenic and thenogenic. They also serve as reservoirs involved in homeostasis, maintenance and cellular regeneration. Their application in bone alterations (traumatic defects, inflammatory and degenerative diseases) has led to the development of new osteo/conductive and/or inductive therapies. During the differentiation process of mscs to the osteogenic lineage, the progression of several cellular states is required to achieve development of the mature cell —osteocyte—, responsible for the balance and regulation of bone formation; mscs can also be differentiated from osteoclasts, which are involved in bone loss (osteoclastogenesis). Different signaling pathways have been proposed to understand the mechanisms of this process of osteogenic differentiation; however, these have not yet been completely understood due to the complexity of signaling pathways and because they converge in common activation points, finally leading the mscs to an osteogenic phenotype.

Keywords: mesenchymal stem cell, differentiation, osteogenesis.

Aarden EM, Burger EH, Nijweide PJ. Function of osteocytes in bone. J Cell Biochem. Jul 1994; 55(3): 287‑99.

Hutchison C, Pilote M, Roy S. The axolotl limb: a model for bone development, regeneration and fracture healing. Bone. Ene 2007; 40(1): 45‑56.

Noonan KJ, Hunziker EB, Nessler J, Buckwalter JA. Changes in cell, matrix compartment, and fibrillar collagen volumes between growth-plate zones. J Orthop Res. Jul 1998; 16(4): 500‑8.

Hall BK, Miyake T. All for one and one for all: condensations and the initiation of skeletal development. Bioessays. Feb 2000; 22(2): 138‑47.

Akiyama H, Chaboissier MC, Martin JF, Schedl A, de Crombrugghe B. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev. Nov 2002; 16(21): 2813-28.

Deschaseaux F, Sensebe L, Heymann D. Mechanisms of bone repair and regeneration. Trends Mol Med. Sep 2009; 15(9): 417-29.

Ferguson C, Alpern E, Miclau T, Helms JA. Does adult fracture repair recapitulate embryonic skeletal formation? Mech Dev. Sep 1999; 87(1‑2): 57‑66.

Albrektsson T, Johansson C. Osteoinduction, osteoconduction and osseointegration. Eur Spine J. Oct 2001; 10 Suppl 2: S96‑101.

Moreau JL, Caccamese JF, Coletti DP, Sauk JJ, Fisher JP. Tissue engineering solutions for cleft palates. J Oral Maxillofac Surg. Dic 2007; 65(12): 2503-11.

Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. Mar 1968; 6(2): 230‑47.

Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. Oct 1970; 3(4): 393‑403.

Caplan AI. Mesenchymal stem cells. J Orthop Res. Sep 1991; 9(5): 641‑50.

Chopp M, Li Y. Treatment of neural injury with marrow stromal cells. Lancet Neurol. Jun 2002; 1(2): 92‑100.

Shih DT, Lee DC, Chen SC, Tsai RY, Huang CT, Tsai CC et al. Isolation and characterization of neurogenic mesenchymal stem cells in human scalp tissue. Stem Cells. Ago 2005; 23(7): 1012‑20.

In ‘t Anker PS, Noort WA, Scherjon SA, Kleijburg-van der Keur C, Kruisselbrink AB, van Bezooijen RL et al. Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential. Haematologica. Ago 2003; 88(8): 845‑52.

Trubiani O, Di Primio R, Traini T, Pizzicannella J, Scarano A, Piattelli A et al. Morphological and cytofluorimetric analysis of adult mesenchymal stem cells expanded ex vivo from periodontal ligament. Int J Immunopathol Pharmacol. Abr‑Jun 2005; 18(2): 213‑21.

Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. Abr 2000; 109(1): 235‑42.

Rebelatto CK, Aguiar AM, Moretão MP, Senegaglia AC, Hansen P, Barchiki F et al. Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue. Exp Biol Med. Jul 2008; 233(7): 901‑13.

Smith JR, Pochampally R, Perry A, Hsu SC, Prockop DJ. Isolation of a highly clonogenic and multipotential subfraction of adult stem cells from bone marrow stroma. Stem Cells. 2004; 22(5): 823‑31.

Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. Jul 2002; 418(6893): 41‑9.

Woodbury D, Schwarz EJ, Prockop DJ, Black IB. Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res. Ago 2000; 61(4): 364‑70.

Petersen BE, Bowen WC, Patrene KD, Mars WM, Sullivan AK, Murase N, Boggs SS, Greenberger JS, Goff JP. Bone marrow as a potential source of hepatic oval cells. Science. May 1999; 284(5417): 1168‑70.

Majumdar MK, Keane-Moore M, Buyaner D, Hardy WB, Moorman MA, McIntosh KR et al. Characterization and functionality of cell surface molecules on human mesenchymal stem cells. J Biomed Sci. Mar‑Abr 2003; 10(2): 228‑41.

Minguell JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med. Jun 2001; 226(6): 507‑20.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4): 315‑7.

Pountos I, Corscadden D, Emery P, Giannoudis PV. Mesenchymal stem cell tissue engineering: Techniques for isolation, expansion and application. Injury. Sep 2007; 38 Suppl 4: S23‑S33.

Owen M. Lineage of osteogenic cells and their relationship to the stromal system. In: Peck WA (ed.) Bone and mineral research. New York: Elsevier; 1985. p. 1-25.

Di Nicola M, Carlo-Stella C, Magni M, Milanesi M, Longoni PD, Matteucci P et al. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. Blood. May 2002; 99(10): 3838‑43.

Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell. May 1997; 89(5): 747‑54.

Siddappa R, Fernandes H, Liu J, van Blitterswijk C, de Boer J. The response of human mesenchymal stem cells to osteogenic signals and its impact on bone tissue engineering. Curr Stem Cell Res Ther. Sep 2007; 2(3): 209‑20.

Caetano-Lopes J, Canhao H, Fonseca JE. Osteoblasts and bone formation. Acta Reumatol Port. Abr‑Jun 2007; 32(2): 103‑10.

Bonizzi G, Karin M. The two NF-kappaB. Activation pathways and their role in innate and adaptive immunity. Trends Immunol. Jun 2004; 25(6): 280‑8.

Hess K, Ushmorov A, Fiedler J, Brenner RE, Wirth T. TNFalpha promotes osteogenic differentiation of human mesenchymal stem cells by triggering the NfkappaB signaling pathway. Bone. Ago 2009; 45(2): 367‑76.

Koenig BB, Cook JS, Wolsing DH, Ting J, Tiesman JP, Correa PE et al. Characterization and cloning of a receptor for BMP-2 and BMP-4 from NIH 3T3 cells. Mol Cell Biol. Sep 1994; 14(9): 5961‑74.

Macias-Silva M, Hoodless PA, Tang SJ, Buchwald M, Wrana JL. Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. J Biol Chem. Oct 1998; 273(40): 25628‑36.

Yamashita H, ten Dijke P, Huylebroeck D, Sampath TK, Andries M, Smith JC et al. Osteogenic protein-1 binds to activin type II receptors and induces certain activin-like effects. J Cell Biol. Jul 1995; 130(1): 217‑26.

Nohno T, Ishikawa T, Saito T, Hosokawa K, Noji S, Wolsing DH et al. Identification of a human type II receptor for bone morphogenetic protein-4 that forms differential heteromeric complexes with bone morphogenetic protein type I receptors. J Biol Chem. Sep 1995; 270(38): 22522‑6.

Abreu JG, Ketpura NI, Reversade B, De Robertis EM. Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta. Nat Cell Biol. Ago 2002; 4(8): 599‑604.

Li CL, Martinez V, He B, Lombet A, Perbal B. A role for CCN3 (NOV) in calcium signalling. Mol Pathol. Ago 2002; 55(4): 250‑61.

Ling L, Nurcombe V, Cool SM. Wnt signaling controls the fate of mesenchymal stem cells. Gene. Mar 2009; 433(1‑2): 1‑7.

Latinkic BV, Mercurio S, Bennett B, Hirst EM, Xu Q, Lau LF et al. Xenopus Cyr61 regulates gastrulation movements and modulates Wnt signalling. Development. Jun 2003; 130(11): 2429‑41.

Huelsken J, Behrens J. The Wnt signalling pathway. J Cell Sci. Nov 2002; 115(Pt 21): 3977‑8.

Wongchuensoontorn C, Liebehenschel N, Schwarz U, Schmelzeisen R, Gutwald R, Ellis E 3rd et al. Application of a new chair-side method for the harvest of mesenchymal stem cells in a patient with nonunion of a fracture of the atrophic mandible--a case report. J Craniomaxillofac Surg. Apr 2009; 37(3): 155‑61.

Logeart-Avramoglou D, Anagnostou F, Bizios R, Petite H. Engineering bone: challenges and obstacles. J Cell

Mol Med. Ene‑Mar 2005; 9(1): 72‑84.

Dong SW, Ying DJ, Duan XJ, Xie Z, Yu ZJ, Zhu CH et al. Bone regeneration using an acellular extracellular matrix and bone marrow mesenchymal stem cells expressing Cbfa1. Biosci Biotechnol Biochem. Oct 2009; 73(10): 2226‑33.

Zhao Z, Yang D, Ma X, Zhao H, Nie C, Si Z. Successful repair of a critical-sized bone defect in the rat femur with a newly developed external fixator. Tohoku J Exp Med. Oct 2009; 219(2): 115‑20.

Kim SJ, Shin YW, Yang KH, Kim SB, Yoo MJ, Han SK et al. A multi-center, randomized, clinical study to compare the effect and safety of autologous cultured osteoblast (Ossron) injection to treat fractures. Bmc Musculoskelet Disord. 2009; 10: doi:10.1186/1471-2474-10-20

Horwitz EM, Prockop DJ, Fitzpatrick LA, Koo WW, Gordon PL, Neel M et al. Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med. Mar 1999; 5(3): 309‑13.

Hernigou P, Poignard A, Beaujean F, Rouard H. Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells. J Bone Joint Surg Am. Jul 2005; 87(7): 1430‑7.

Mesimäki K, Lindroos B, Törnwall J, Mauno J, Lindqvist C, Kontio R et al. Novel maxillary reconstruction with ectopic bone formation by gmp adipose stem cells. Int J Oral Maxillofac Surg. Mar 2009; 38(3): 201‑9.

Hibi H, Yamada Y, Ueda M, Endo Y. Alveolar cleft osteoplasty using tissue-engineered osteogenic material. Int J Oral Maxillofac Surg. Jun 2006; 35(6): 551‑5.

Mesenchymal stem cells: an alternative for bone regeneration. (2014). Revista Nacional De Odontología, 8(15), 79-86. https://revistas.ucc.edu.co/index.php/od/article/view/275

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Mesenchymal stem cells: an alternative for bone regeneration. (2014). Revista Nacional De Odontología, 8(15), 79-86. https://revistas.ucc.edu.co/index.php/od/article/view/275

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Mesenchymal stem cells : an alternative for bone regeneration

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