Extrarradicular biofilm
a clinical case report
Biofilm is a group of cells sticking together on a surface, embedded within a matrix of exopolysaccharides protecting them from antibiotics. Over 60% of microbial infections are caused by biofilms. The antimicrobial resistance increase of these communities involve several mechanisms, including: inactivation of antibiotics through extracellular polymers, decline of the growth rate by nutrient limitation and phenotypic changes in the bacterial cells, as a result of the resistant gene acquisition within the biofilm. Prognosis for treatment and eradication of biofilms is not the best one. The development of new medicines becomes necessary and urgent to counter biofilm related infections. This post shows the case of a 44 year old woman who goes to see the doctor for pain in 12, particularly upon vertical percussion. Such episodes are caused by under extended endodontics of 12 and in the extraradicular filling material of ducts. After the x-ray evaluation and appropriate application of the treatment plan, spherical shaped bacteria were detected within the biofilm, which caused the patient’s infection.
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Siqueira J. Aetiology of root canal treatment failure: why well-treated teeth can fail. Int Endodon J. 2001;34(1):1-10.
Siqueira J, Lopes H. Bacteria on the apical root surfaces of untreated teeth with periradicular lesions: a scaning electron microscopy study. Int endodon J. 2001;34:216-20.
George S, Kisben A, Song K. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod. 2005;31(12):867-72.
Nair R. Light and electron microscopic studies of root canal flora and periapical lesions. J Endod. 1987;13(1):29-39.
Molven O, Olsen I, Kerekes K. Scanning electron microscopy of bacteria in the apical part of root canals in permanent teeth with periapical lesions. Endod Dent Traumatol. 1991;7:226-9.
Distel J, Hatton J, Gillespie J. Biofilm formation in medicated root canals. J Endod. 2002;28(10):689-93.
Clegg M, Vertucci F, Walker C, Belanger M, Britto L. The effect of exposure to irrigant solutions on apical dentin biofilm in vitro. J Endod. 2006; 32(5):434-7.
Tronstad L, Barnett F, Cervone F. Periapical bacterial plaque in teeth refractory to endodontic treatment. Endodon Dent Traumatol. 1990;6:73-77.
Lomcali G, Sen B, Cankaya H. Scanning electron microscopic observations of apical root surfaces of teeth with apical periodontitis. Endod Dent Traumatol. 1996;12:70-6.
Walton R, Ardjmand K. Histological evaluation of the presence of bacteria in induced periapical lesions in monkeys. J Endod. 1992;18(5):216-21.
Noiri Y, Ehara A, Kawahara T, Takemura N, Ebisu S. Participation of bacterial biofilms in refractary and chronic periapical periodontitis. J Endod. 2002;28(10):679-83.
Leonardo M, Rossi M, Silva L, Ito I, Bonifácio K. EM evaluation of bacterial biofilm and microorganism on the apical external root surface of human teeth. J Endod. 2002;28(12):815-8.
Sunde P, Olsen I, Debelian G, Tronstad L. Microbiota of periapical lesions refractory to endodontic therapy. J Endod. 2002;28(4):304-10.
Nair R, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after "one-visit" endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon. 2005;99:231-52.
Nair R, Sjögren U, Krey G, Kahnberg K, Sundqvist G. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endodon. 1990;16(12):580-8.
Haapasalo M, Endal U, Zandi H, Coil J. Eradication of endodontic infection by instrumentation and irrigation solutions. Endodontics Topics. 2005;10:77-102.
Haapasalo M. Control and elimination of endodontic infection. Roots. 2006;1:15-22.
Jefferson K. What drives bacteria to produce a biofilm? Microbiology letters. 2004; 236(2):163-73.
Donlan R, Costerton W. Biofilms: survival mechanisms of clinically relevant microorganisms. Clinical Microbiology Reviews. 2002;15(2):167-93.
Costerton J, Cheng G, Geesey T, Ladd J, Nickel M, Dasgupta M, Marrie T. Bacterial biofilms in nature and disease. Annu Rev Microbiol. 1987; 41:435-64.
Gilbert P, Das J, Foley I. Biofilms susceptibility to antimicrobials. Adv Dent Res. 1997;11:160-67.
Tronstad L, Barnett F, Riso K, Slots J. Extraradicular endodontic infections. Endod Dent Traumatol. 1987;3:86-90.
Tronstad L, Kreshtool D, Barnett F. Microbiological monitoring and results of treatment of extraradicular endodontic infection. Endod Dent Traumatol. 1990;6:129-36.
Costerton J, Lewandowski Z, Caldwell D, Korber D, Lappin-Scott H. Microbial biofilms. Annu Rev Microbiol. 1995;49:711-45.
Davey M, O´toole G. Microbial biofilms: from Ecology to Molecular Genetics. Microbiol Mol Biol Rev. 2000;64(4):847-67.
Lasa I, Del Pozo J, Penaldes J, Leiva J. Bacterial biofilms and infection. An Sist Sanit Navar. 2005; 28(2):163-75.
Lasa I, Del Pozo J, Penaldes J, Leiva J. Bacterial biofilms and infection. An Sist Sanit Navar. 2005;28(2):163-75.
Bowden G, Li Y. Nutritional influences on biofilm development. Adv Dent Res. 1997;11(1):81-99.
