Efecto del cambio de jaulas en la producción de ratas Sprague Dawley

Edwin León; Ledis Reyes; Luis Pacheco; Fátima Caiza

doi:10.16925/sp.v9i19.705

Effects of Cage Change on the Production of Sprague Dawley Rats

Investigación

https://doi.org/10.16925/sp.v9i19.705

Edwin León Ver Biografía

Universidad de Costa Rica

Ledis Reyes Ver Biografía

Universidad de Costa Rica

Luis Pacheco Ver Biografía

Universidad de Costa Rica

Fátima Caiza Ver Biografía

Universidad de Costa Rica

Olfactory signals that enable mother rats to communicate with their young are critical. More frequent cleaning of primary enclosures eliminates olfactory signals that affect the number of pups weaned. To measure the influence of changes on the production of rats of the Sprague Dawley strain, it was decided to change the cages of females once a week throughout the production cycle (from mating until weaning) (28 females) for the experimental group, and twice a week (28 females) for the control group because this is the usual system for changing them. The minimum and maximum temperature and humidity values of the secondary enclosure were also recorded to compare their influence on changes in the animals; this was then compared to the effect on the number of births or the change in the system (8 females in each group). The average number of pups born to mother rats was compared using hypothesis contrasting and the effects of temperature, humidity and a second delivery were analyzed using logistical regression. It was found that when performing hypothesis testing, there was no statistically significant difference in the number of animals produced at weaning due to one change or two changes. And when compared to the other factors, it was found that the number of females produced in the logistical regression was more significant than the modification in changing the animals’ cages.

Keywords: frecuencia de cambio de jaulas, producción animal de laboratorio, recinto primario, Sprague Dawley

Burn CC, Mason GJ. Absorbencies of six different rodent beddings: commercially advertised absorbencies are potentially misleading. Lab Anim. Ene 2005; 39(1): 68‑74.

Health and Safety Executive. Control of Sub-stances Hazardous to Health (coshh) Approved Codes of Practice. London: HMSO; 1994.

Broderson JR, Lindsey JR, Crawford JE. The role of environmental ammonia in respiratory mycoplasmosis of rats. Am J Pathol. 1976; 85(1): 115‑130.

Gamble MR, Clough G. Ammonia build-up in animal boxes and its effect on rat tracheal epithelium. Lab Anim. Abr 1976; 10(2): 93-104.

Serrano LJ. Carbon dioxide and ammonia in mouse cages:effects of cage covers, population, and activity. Lab Anim Sci. Feb 1971; 21(1): 75‑85.

Van Winkle TJ, Balk MW. Spontaneous corneal opacities in laboratory mice. Lab Anim Sci. Jun 1986; 36(3): 248‑55.

Berg RW, Buckingham K, Stewart RL. Etiologic Factors in Diaper Dermatitis: The Role of Urine. Pediatr Dermatol. Feb 1986; 3(2): 102‑6.

St Claire MB, St Claire MC, Davis JA, Chang L, Miller GF. Barrier ﬁlm protects skin of incontinent rats. Contemp Top Lab Anim Sci. Sep 1997; 36(5): 46‑8.

Burn C, Peters A, Day MJ, Mason GJ. Long-term effects of cage-cleaning frequency and bedding type on laboratory rat health, welfare, and handleability: a crosslaboratory study. Lab Anim. Oct 2006; 40(4): 353‑70.

Chantry D. The effects of handling and cage changing on breeding mice. J Ani Tech Ass. 1964; 15: 78‑80.

Reeb-Whitaker CK, Paigen B, Beamer WG, et al. The impact of reduced frequency of cage changes on the health of mice housed in ventilated cages. Lab Anim. Ene 2001; 35(1): 58‑73.

Peters AG, Bywater PM, Festing MFW. The effect of daily disturbance on the breeding perfor-mance of mice. Lab Anim. Abr 2002; 36(2): 188‑92.

Cisar CF, Jayson G. Effects of frequency of cage cleaning on rat litters prior to weaning. Lab Anim Care. 1967; 17: 215‑17.

Burn C, Mason G. Effects of cage-cleaning frequency on laboratory rat reproduction, cannibalism, and welfare. Appl Anim Behav Sci. Nov 2008; 114(1); 235‑247.

Ministerio de Ciencia y Tecnología. Guía para el manejo de animales de laboratorio: Comité Técnico Nacional sobre Utilización de Animales de Laboratorio. 1998; La Gaceta. (44).

León, E., Reyes, L., Pacheco, L., & Fátima. (2013). Effects of Cage Change on the Production of Sprague Dawley Rats. Spei Domus, 9(19), 17-22. https://doi.org/10.16925/sp.v9i19.705

Download Citation

En calidad de autor del artículo, declaro que este, es un trabajo original, inédito, de mi creación exclusiva, que no se ha postulado a evaluación simultánea en otra publicación y que no cuenta con algún impedimento de cualquier naturaleza para la concesión de los derechos previstos en este contrato.

En ese sentido, me comprometo a esperar un resultado de evaluación de la revista, antes de considerar su presentación a otro medio, en caso de que la respuesta de publicación no sea positiva; adicionalmente, me comprometo a responder por cualquier acción de reivindicación, plagio u otra clase de reclamación que al respecto pudiera sobrevivir por parte de terceros.

Asimismo, declaro que como autor o coautor, estoy de acuerdo por completo con los contenidos presentados en este trabajo y cedo todos los derechos patrimoniales, es decir, la reproducción, comunicación pública, distribución, divulgación, transformación, puesta a disposición y demás formas de explotación de la obra por cualquier medio o procedimiento, a la revista y a la Editorial de la Universidad Cooperativa de Colombia.

Issue

Vol. 9 No. 19 (2013)

Published

2013-12-01

Downloads

PDF (Spanish)

How to Cite

León, E., Reyes, L., Pacheco, L., & Fátima. (2013). Effects of Cage Change on the Production of Sprague Dawley Rats. Spei Domus, 9(19), 17-22. https://doi.org/10.16925/sp.v9i19.705

Download Citation

Metrics

##plugins.generic.paperbuzz.sourceName.fileDownloads##

882

https://plu.mx/plum/a/?doi=10.16925/sp.v9i19.705