• Investigación

    Estandarización de la citología vaginal exfoliativa correlacionando los niveles séricos de progesterona en perras durante la peri-ovulación.

    Vol. 1 No. 2 (2005)
    Published: 2026-07-05
    Victor Hernan Arcila Quiceno
    Cesar Augusto Serrano Novoa
    Milena Hernandez
    Luz Piedad Meza

    Because accuracy of cytology as definitive test to determine the period of ovulation in canine females (dogs) it is controversial, it makes necessary to correlate typical characteristics of ovulation, as plasmatic levels of progesterone and cytogical changes to ensure optimus time of ovulation. Progesterone levels measures were carried out by ELISA test, to conclude if superficial cells percent found in vaginal cytology, is related with the hormonal levels during ovulation. Cytologic and serum samples of 30 healthy female dogs were collected every 48 hours. The datas were analysed by correlation test. When progesterone levels were below 10 ng/ml, the percentage of superficial cells was 72 +/- 1,72%, while the percentage of in flake, queratinizadas, intermediate cellular types were 13 +/- 3,12%, 84 +/- 1,14% and 16 +/- 2,62% respectively. When progestrone levels were between 10 to 20 ng/ml, the percentages of superficial cells was 68 +/- 2,08%, while in flake, queratinizadas, and intermediate cellular types were 11 +/- 3,19%, 80 +/- 1,9% and 21 +/- 3,63% respectively. Finally, within highest ranges (>20 ng/ml), the percentages of superficial cells was 57 +/- 3,52%, while in flake, queratinizadas and intermediate cells were 10 +/- 1,35%, 64+/- 2,75% and 35 +/- 3,31% respectively.

    Keywords: Array, Array, Array, Array, Array

    How to Cite

    Arcila Quiceno, V. H., Serrano Novoa, C. A., Hernandez, M., & Meza, L. P. (2005). Estandarización de la citología vaginal exfoliativa correlacionando los niveles séricos de progesterona en perras durante la peri-ovulación. Spei Domus, 1(2). https://revistas.ucc.edu.co/index.php/sp/article/view/565

    Feldman N. Endocrinología y reproducción en perros y gatos. 2ª ed., México: McGraw-Hill, 2000. pp. 572-592.[2] Esquibel Lacroix C. en Primer seminario de endocrino-logía con énfasis en reproducción. Abril 29-30, mayo 1°. 2000.

    Bren de Arguello Nuri. Citología diagnóstica vaginal. Editorial Manual Médico: México, 2001. pp. 19-30.

    Linde C., Karlsson IJ. Small animal pract. 1984, 2577.

    Concannon PW, Hansel W, Visek W. Biol Reprod 1975; 13, 112.

    Bouchar GF, Malugani N, Younquist RS, Krause GF, Concannon PW, et al. Determination of ovulation in the bitch with a qualitative progestorone enzymeinmu-noassay in serum, plasma and whole blood. J Reprod Fertilsuppl 1993; 47:517-8.

    Goodman M. Ovulation timing. Concepts and contro-versies. Vet Clin North Am Small Animal Pract 2001; (2): 219-235.

    Hayer P, Gunzel-Apel AR, Luerssen D, Hoppen HO. Ultrasonographic monitorin of follicular development, ovulation and the early luteal phasej in the bitch. J Re-prod Fertile 1993; 47:93-100.

    Renton JP, Boyd JS, Harvey MJ, Fergunson JM, Nickson DA, Eckersall PD. Comparison of endocrine changes and ultrasound as means of identifying ovulation in the bitch. Res Vet Sci Jul 1992; 53 (1):74-9.

    Hay MA, King WA, Gartley CJ, Goodrowe KL. Corre-lation of periovulation serum and fecal progestins in the domestic dog. Can J Vet Res Jan 2000; 64(1):59-63.

    Kaneco J., Harvey JW and Bruss ML. Clinical Bio-chemistry of Domestic Animals. Fifth Edition. Acade-mic Press; 1997.

    Bonagura K. Terapéutica veterinaria de pequeños ani-males. XII. McGraw Hill Interamericana. pp. 1170-1171.

    Simpson JMG, England GCW, JarveyMJ. Manual de reproducción y neonatología en pequeños animales. Madrid: Editorial Harcourt S.A.; 2000, pp. 2-5.

    Hiemstra M, Schaefers-Okkens AC, Teske E, Koois-tra HS. The reablity of vaginal cytology in determining the optimal mating time in the bitch. Tijdschr Dierge-neeskd. Nov 2001; 126(21):685-9.

    Root K. Uso de kits comerciales para ensayos de hor-mona luteinizante y de progesterona en el manejo re-productivo canino. Ithaca, New York. USA. 2001. URL: www.ivis.org.

    Van Arrle P, Aguer D, Baars J y otros. Compedium de reproducción animal. 3ª. Edición. Laboratorios Inter-vet. S. A. 1999. pp. 125-143.

    Ortega Pacheco A, Ramírez Buenfil JC, Leal Ortega JA. Actividad estral de perras callejeras en la ciudad de Mérida, Yucatán y su relación con edad, tamaño y condición corporal. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Mé-rida, Yucatán, México. Revista Biomédica. Vol. 11/No. 2/Abril-Junio, 2000. URL: www.uady.mx/~biomedic/rb001124.pdf

    Citología vaginaI exfoliativa en perras con fines de diag-nóstico de la fase estrual (celo principalmente). Revista de la Facultad de Medicina Veterinaria y Zootecnia. Univer-sidad de San Carlos de Guatemala; 2000; 15(1):10-14..

    MÉTRICAS
    ARTICLE VIEWS: 1229
    PDF VIEWS: 4083