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  • Investigación

    Influence of oviduct cell secretions cultured in vitro on the viability of frozen-thawed bovine sperm cells

    DOI: https://doi.org/10.16925/sp.v11i22.1158
    Vol. 11 No. 22 (2015)
    Published: 2015-06-01
    Patrícia Valleriote
    Carla Paes de Carvalho
    Diego Fernando Dubeibe
    Edgar Mauricio Mogolllón
    Angelo Jose Burla
    Introduction: in cattle, the oviduct is essential in the process of maturation, fertilization and acquisition of competence for proper development of an embryo until the blastocyst stage. Once the cells in the internal lining of the oviduct provide substances that positively affect sperm as it crosses the female genital tract, the surface components are modified or removed by secretions present therein. Such modifications may include cholesterol depletion on sperm surface, altered glycosaminoglycans, and change in ions. Methodology: the objective was to evaluate the influence of adding cell culture medium of bovine oviduct cells on sperm parameters such as motility, vigor, and integrity of cytoplasmic membrane. The sperm cells were incubated with 164 μl of Talp-sp-Uso in the control group (TCt); 161.5μl of Talp-sp-Uso, in addition to 2.5μl of heparin (20μg/ml) in treatment 1 (THep) and 128μl of Talp-sp-Uso, along with 36μl of culture medium with secretions of bovine oviduct cells in treatment 2(TOv). Tests were performed at zero time (to show that the medium of treatments did not hurt the sperm cells) and after three hours of incubation. Results: no significant differences in progressive motility, vigor and integrity of cell membrane between treatments were found. However, sperm cells cultured with oviduct cell secretions showed a greater percentage of total motility after three hours of culturing, in contrast to sperm cultured in the presence of heparin. Conclusion: oviduct cell secretions do not affect progressive motility, vigor or integrity of the cytoplasmic membrane of sperm cells; however, they prolong sperm motility.
    Keywords: Array, Array, Array
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    How to Cite

    Valleriote, P., Paes de Carvalho, C., Dubeibe , D. F., Mogolllón , E. M., & Burla , A. J. (2015). Influence of oviduct cell secretions cultured in vitro on the viability of frozen-thawed bovine sperm cells. Spei Domus, 11(22). https://doi.org/10.16925/sp.v11i22.1158

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    References

    Areekijseree, M. And Veerapraditsin, T. Characterization of porcine oviductal epithelial cells, cumulus cells and granulosa cells-conditioned media and their ability to induce acrosome reaction on frozen-thawed bovine spermatozoa. Micron. 2008; v.39 (2): 160- 167.

    Hunter, R. H. Components of oviduct physiology in eutherian mammals. Biological Reviews of the Cambridge Philosophical Society. 2012.; v. 87 (1): 244–255.

    Ghersevich, S; Massa, E Y Zumoffen, C. Oviductal secretion and gamete interaction. Reproduction. 2015; v.149: 1–14.

    Coy, P; García-Vázquez, F.A; Visconti, P. E; Avilés, M. Roles of the oviduct in mammalian fertilization. 2012; v.144: 649–660.

    Pereira, R. J; Tuli, R. K; Wallenhorst, S; Holtz, W. The Effect of Heparin, Caffeine and Calcium Ionophore A23187 on in vitro Induction of the Acrosome Reaction in Frozen-Thawed Bovine and Caprine Spermatozoa. Theriogenology. 2000; v. 54 (2): 185-192.

    Junqueira, L.C; Carneiro, J. Histologia básica. 10ª edição. Rio de Janeiro: Editora Guanabara Koogan; 2004.

    Aguilar, J.; Reyley, M. The uterine tubal fluid: secretion, composition and biological effects. Animal Reproduction. 2005; v.2: 91-105.

    Walter, I.; Bavdek, S. Lectin binding patterns of porcine oviduct mucosa and endometrium during the oestrous cycle. Journal of Anatomy. 1997; v. 190: 299-307.

    Kamaci, M; Suludere, Z; Irmak, K; Can, C; Bayhan, H. Observation of isthmic epithelial cells from fallopian tubes at follicular phase by light and scanning electron microscope. Eastern Journal of Medicine. 1999; v. 4 (2): 51-53.

    Leese, H.J; Tay, J.I; Reischl, J; Downing, S.J. Formation of Fallopian tubal fluid: role of a neglected epithelium. Reproduction. 2001; v. 121 (3): 339-346.

    Lapointe, J; Bilodeau, J.F. Antioxidant defenses are modulated in the cow oviduct. Biology of Reproduction. 2003; v. 68 (4): 1157-1164.

    Grippo, A. A.; Way, A.L.; Killian, G.J. Effect of bovine ampullary and isthmic oviductal fluid on motility, acrosome reaction and fertility of bull spermatozoa. Journal of Reproduction and Fertility. 1995; v. 105 (1): 57-64.

    Killian, G.J. Evidence for the role of oviduct secretions in sperm function, fertilization and embryo development. Animal Reproduction Science. 2004; v. 82: 141-153.

    Grippo, A; Luo, Y;, Rougeau, B And Wyatt,W. Monosaccharides are not detected in whole or isthmic bovine oviductal fluid collected throughout the estrous cycle, as analyzed by HPLC. Theriogenology. 2000; v. 53 (3):717-726.

    Locatelli, Y; Cognié, Y; Vallet, J.C; Baril, G; Verdier, M; Poulin, N; Legendre, X; Mermillod, P. Successful use of oviduct epithelial cell coculture for in vitro production of viablered deer (Cervus elaphus) embryos. Theriogenology. 2005; 64 (8): 1739-1739.

    Buhi, W.C. Characterization and biological roles of oviduct-specific, oestrogen-dependent glycoprotein. Reproduction. 2002; v.123 (3): 355-362.

    Leese, H.J. The formation and function of oviduct fluid. Journal of Reproduction and Fertility. 1988; v.82: 843-856.

    Gandolfi, F.; Brevini, J. A.; Richardson, L.; Brown, C.R.; Moor, R.M. Characterization of proteins secreted by sheep oviduct epithelial cells and their function in embryonic development. Development. 1989; v. 106 (2): 303-312.

    Yanagimachi, R. Mammalian fertilization. Em: Knobil, E. E Neill J.D. The Physiology of Reproduction. 2da edição. Nova York: Raven Press; 1994. 189-317.

    Flesch, F.M.; Gadella, B.M. Dynamics of the mammalian sperm plasma membrane in the process of fertilization. Biochimica et Biophysica Acta. 2000; v.1469 (3): 197-235.

    Roldan, E.R.S; Gomendio, M. Morphological, functional and biochemical changes underlying the preparation and selection of fertilizing spermatozoa in vitro. Animal Reproduction Science. 1992; v.28: 69-78.

    Gordon, I. Laboratory Production of Cattle Embryos. 1ra edición. Londres: CAB International. Wallingford; 1994.

    Pérez, L.J; Valcarcel, A; De Las Heras, M. A; Moses, D. F; Baldassare, H. In vitro capacitation and induction of acrossomal exocytosis in ram spermatozoa as assessed by the chlortetracycline assay. Theriogenology. 1996; v. 45 (5): 1037-1046.

    Perez, L.J.; Valcárcel, A.; Heras, M.A.; Baldassarre, H. Comparative study of four techniques for evaluation of sperm quality in ovine and bovine frozen thawed samples. Reproduction in Domestic Animal. 1997; v.32: 157-160.

    Suarez S.S. Regulation of sperm storage and movement in the mammalian oviduct. Int J Dev Biol. 2008; v.52 (5–6): 455–462.

    Hafez, E.S.E. Reprodução Animal. 7ª edição. São Paulo: Ed. Manole Ltda; 2004.

    Buffone, M. G; Hirohashi, N; Gerton, G, L. Unresolved Questions Concerning Mammalian Sperm Acrosomal Exocytosis. Biology of Reproduction. 2014; v.90 (5):112: 1–8.

    Quintero, I; Ghersevich, S; Caille, A; Munuce, M; Daniele, S And Lida Morisol. Effects of human oviductal in vitro secretion on spermatozoa and search of sperm–oviductal proteins interactions. International Journal of Andrology. 2005; 28 (3): 137–143.

    Parrish, J.J. Bovine in vitro fertilization: in vitro oocyte maturation and sperm capacitation with heparin. Theriogenology. 2014; v.81 (1): 67-73.

    Valleriote, P.S; Dias, A.J.B; Paes De Carvalho, F; Paes Sobrinho, C. Criopreservação de sêmen ovino em solução de trealose. Acta Scientiae Veterinariae. 2005; v.33, Sup l.1:.310

    Correa,J.R; Pace, M.M; Zavos, P.M. Relationships among frozen-thawed sperm characteristics assessed via the routine semen analysis, sperm functional test and fertility of bulls in an artificial insemination program. Theriogenology. 1997; v.48:721-203.

    Pertoft, H; Laurent, T.C; Laas, T; Kagedal, L. Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll). Analytical Biochemistry. 1978; v. 88 (1): 271-282.

    Zúccari, C.E; Carrijo, P.R; Leite, P.A; Scaldelai, P.R; Rodovalho, N. C; Zanenga, C.A; Kiefer, C; Costa E Silva, E.V. Seleção em gradiente de Percoll® sobre os parâmetros espermáticos do sêmen bovino congelado. Rev. Bras. Saúde Prod. An. 2008; v.9 (2): 358-366.

    Kumaresan, A; Ansari, M.R; Garg, A. Modulation of post-thaw sperm functions with oviductal proteins in buffaloes. Animal Reproduction Science. 2005; v. 90 (1-2): 73-84.

    Yao Y.Q; Ho P.C; Yeung W.S. Human oviductal cells produce a factor(s) that maintains the motility of human spermatozoa in vitro. Fertility and Sterility. 2000; v. 73 (3):479–486.

    Boquest A. C., Smith J. F, Briggs R. M., Duganzich D. M And. Summers P. M ; Effects of bovine oviductal proteins on bull spermatozoal function. Theriogenology. 1999; v.51 (3): 583-595.

    Imam, S; Ansari, M.R; Kumar, A; Singh, C; Bharti, V.K; Kumaresan, A. Effect of Oviductal Proteins on Structural and Functional Characteristics of Cryopreserved Sperm in Murrah Buffaloes. Reprod. Dom. Anim. 2010; v. 45 (2): 302-306.

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