SMU.759 encoding a collagenase-like protease is constitutive in Streptococcus mutans
Introduction: type I collagen is found in dentin, which can be degraded by enzymatic action. The SMU.759 gene of Streptococcus mutans, serotype c, encodes a protease with possible collagenase activity, probably affecting this type of collagen and favoring the progression of caries, since, in processes after demineralization, type I collagen in dentin is exposed to the action of this bacteria. The aim is to determine if there are differences in the presence or absence of SMU.759 in isolates from patients with and free of caries.
Materials and methods: isolates from a strain were used from schoolchildren between 6 and 9 years of age with and free of caries from the city of Medellín, Colombia. The primers for SMU.759 were designed, PCR was performed, the amplified ones were observed on a 1.2% agarose gel.Results: SMU.759 was amplified in all the isolates used.
Conclusion: the SMU.759 gene, which encodes a protease, would be a constitutive gene of the S. mutans genome
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