DOI: https://doi.org/10.16925/od.v14i27.2338

Antifungal effect of polymeric films containing the essential oil of Schinus terebinthifolius on Candida albicans biofilms

Hamdia A. Kassim, Karina Scheuermann, Alinne D. M. Gomes, Rubén D. Sinisterra, Maria E. Cortés

Resumen


Introduction: Candida albicans is a commensal microorganism in humans, that may cause oral candidiasis in people with immune deficiencies or ill-fitting dentures. C. albicans is becoming more resistant to drugs used in its treatment. This makes it necessary to develop new medicines that do not damage the host cells. There are many studies on the antimicrobial activity of leaves, stem and roots of Schinus terebinthifolius, but few studies attend its fruits. The aim of this study is to investigate the antifungal effect of polymeric films of hydroxypropyl methylcellulose (hpmc) containing the essential oil of the fruits of S. terebinthifolius against biofilms of C. albicans. 

Materials and methods: The oil was prepared from the fruits of S. terebinthifolius and incorporated into polyme- ric films of hpmc using concentrations of 0.146, 0.073 e 0.037 g.mL-1. The minimum inhibitory concentration  (mic) was determined by micro dilution, wherein the minimum inhibitory concentration was 0.78%. The antifun- gal effect of the films was tested using agar in petri dishes and acrylic resin blocks, both contaminated with  C. albicans.

Results: The polymeric films containing essential oil had average inhibition zones of 24 mm, 21 mm and 20 mm for the concentrations 0.037, 0.073 and 0.146 g.mL1, respectively.

Conclusion: The treated resin blocks showed no fungal growth afterwards, with the exception of the control blocks. The use of antimicrobial films containing natural products like S. terebinthifolius in the treatment of diseases such as oral candidiasis associated to prosthetic seems promising and with a decreased possibility of side effects.


Palabras clave


candidiasis, biological products, antifungal agents, oils, anacardiaceae, microbial sensitivity tests, dentures.

Texto completo:

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Referencias


Sholapurkar AA, Pai KM, Rao S. Comparison of efficacy of fluconazole mouthrinse and clotrimazole mouthpaint in the treatment of oral candidiasis. Aust Dent J. 2009;54:341-6. doi: http://dx.doi.org/ 10.1111/j.1834-7819.2009.01160.x

Burgers R, Hhnel S, Reichert TE, Rosentritt M, Behr M, Gerlach T, et al. Adhesion of Candida albicans to various dental implant surfaces and the influence of salivary pellicle proteins. Acta Biomater. 2010;6:2307-313. doi: http://dx.doi.org/10.1016/j. actbio.2009.11.003

Shulman JD, Rivera-Hidalgo F, Beach MM. Risk factors associated with denture stomatitis in the United States. J Oral Pathol Med. 2005;34:340-6. doi: http:// dx.doi.org/10.1111/j.1600-0714.2005.00287.x

Figueiral MH, Azul A, Pinto E, Fonseca PA, Branco FM, Scully C. Denture-related stomatitis, identification of etiological and predisposing factors a large cohort. J Oral Rehabil. 2007;34:448-55. doi: http:// dx.doi.org/10.1111/j.1365-2842.2007.01709.x

Gomez-Pino MF, Silva-Rios MA, Paez-Herrera MC, Gomez-Ortega J, Zea-Restrepo FJ, Agudelo Suarez AA. Salud bucal y factores relacionados en pacientes portadores de protesis bimaxilar en una institucion docencia servicio de Medellin, Colombia. Rev Nac Odontol. 2017;13(24):65-77. doi: https:// doi.org/10.16925/od.v12i24.1662. Available from: https://revistas.ucc.edu.co/index.php/od/article/ view/1662

Parvinen T. Stimulated salivary flow rate, pH and Lactobacillus and yeast concentrations in persons with different types of dentition. Scand J Dent Res. 1984;92:412-18. doi: http://dx.doi.org/ 10.1111/j.1600-0722.1984.tb00910.x

Nevzatoğlu EU, Ozcan M, Kulak-Ozkan Y, Kadir T. Adherence of Candida albicans to denture base acrylics and silicone-based resilient liner materials with different surface finishes. Clin Oral Investig. 2007;11(3):231-6. doi: https://doi.org/10.1007/ s00784-007-0106-3

Johann S, Pizzolatti MG, Donnici CL, Resende MA. Antifungal properties of plants used in Brazilian traditional medicine against clinically relevant fungal pathogens. Braz J Microbiol. 2007;38:632-7. doi: https://doi.org/10.1590/s1517-83822007000400010

Lima MRF, Luna JS, Santos AF, Andrade MCC, Sant´ Ana AEG, Genetj J, et al. Anti-bacterial activity of some Brazilian medicinal plants. J Ethnopharmacol. 2006; 105:137-47. doi: http://dx.doi.org/10.1016/j. jep.2005.10.026

Correa MP, Pena LA. Dicionario de plantas uteis do Brasil e das exoticas cultivadas. Rio de Janeiro: Ministerio da Agricultura, Instituto Brasileiro de Desenvolvimento Florestal; 1984.p. 1874-1934

Correia SJ, David JP, David JM. Metabolicos secundarios de especies de Anacardiaceae. Quim Nova. 2006;29:1287-300. doi: https://doi.org/10.1590/ s0100-40422006000600026

Barbosa LCA, Demuner AJ, Clemente AD, Paula VF, Ismail FMD. Seasonal variation in the composition of volatile oils from Schinus terebinthifolius Raddi. Quim Nova. 2007;30:1959-965. doi: https://doi. org/10.1590/S0100-40422007000800030

Carvalho MG, Melo AGN, Aragao CFS, Raffin FN, Moura TFAL. Schinus terebinthifolius Raddi: chemical composition, biological properties and toxicity. Rev Bras Plantas Med. 2013;15(1):158-169. doi: https:// doi.org/10.1590/S1516-05722013000100022

Bendaoud H, Romdhane M, Souchard JP, Cazaux S, Bouajila J. Chemical composition and anticancer and antioxidant activities of Schinus molle L. and Schinus terebinthifolius Raddi berries essential oils. J Food Sci. 2010;75:466-72. doi: https://doi.org/ 10.1111/j.1750-3841.2010.01711.x

Azevedo CF, Quirino ZGM, Bruno RLA. Estudo farmacobotanico de partes aereas vegetativas de aroeira-vermelha (Schinus terebinthifolius Raddi, Anacardiaceae). Rev Bras Plant Med, 2015:17(1),26- 35. Mar. https://doi.org/10.1590/1983-084x/11_090

Coutinho HI, Lopes OLS, Torres OMJ, Matias JEF, Coelho JCU, Stahlke JHJ, et al. Schinus terebinthifolius Raddi and it’s influence in the healing process of colonic anastomosis. Experimental study in rats. Acta Cir Bras. 2006;21:9-54. http://dx.doi. org/10.1590/S0102-86502006000900008

Santos EB, Dantas GS, Santos HB, Diniz MFF, Melo, Sampaio FC. Estudo etnobotanico de plantas medicinais para problemas bucais no municipio de Joao Pessoa, Brasil. Rev Bras Farmacog. 2009;19(1b):321-4. https://doi.org/10.1590/S0102- 695X2009000200024

Enioutina EY, Salis ER, Job KM, Gubarev MI, Krepkova LV, Sherwin CM. Herbal Medicines: challenges in the modern world. Part 5. status and current directions of complementary and alternative herbal medicine worldwide. Expert Rev Clin Pharmacol. 2017 Mar;10(3):327-38. doi: https://doi.org/ 10.1080/17512433.2017.1268917.

Schmourlo G, Mendonca-Filho RR, Alviano CS, Costa SS. Screening of antifungal agents using etanol precipitation and bioautography of medicinal and food plants. J Ethnopharmacol. 2005;96:563-8. doi: https://doi.org/10.1016/j.jep.2004.10.007

Medeiros KCP, Monteiro JC, Diniz MFFM, Medeiros IA, Silva BA, Piuvezam MR. Effect of the activity of the Brazilian polyherbal formulation, Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Raddi in inflammatory models. Rev Bras Farmacogn. 2007;17:23-8. doi: https://doi. org/10.1590/S0102-695X2007000100006

Cavalher-Machado SC, Rosas EC, Brito FA, Heringe AP, Oliveira RR, Kaplan MA, Figuet al. The antiallergic activity of the acetate fraction of Schinus terebinthifolius leaves in IgE induced mice paw edema and pleurisy. Int Immunopharmacol. 2008; 8:1552-60. doi: https://doi.org/10.1016/j.intimp.2008. 06.012

Pereira EM, Gomes RT, Freire NR, Aguiar EG, Brandao MG, Santos VR. In vitro antimicrobial activity of Brazilian medicinal plant extracts against pathogenic microorganisms of interest to dentistry. Planta Med. 2011;77:401-4. doi: https://doi.org/ 10.1055/s-0030-1250354

Oliveira Junior LFG, Santos RB, Reis FO, Matsumoto ST, Bispo WMS, Machado, LP et al. Efeito fungitoxico do oleo essencial de aroeira da praia (Schinus terebinthifolius raddi) sobre Colletotrichum gloeosporioides. Rev Bras Pl Med. 2013;15:150-7. doi: https://doi.org/10.1590/S1516-05722013000100021

Silva AB, Silva T, Franco ES, Rabelo SA, Lima ER, Mota RA, et al. Antibacterial activity, chemical composition and cytotoxicity of leaf´s essential oil from Brazilian pepper tree (Schinus terebinthifolius, raddi). Braz J Microbiol. 2010;41:158-63. doi: https:// doi.org/10.1590/s1517-83822010000100023

Atti Dos Santos AC, Rossato M, Serafini LA, Bueno M, Crippa LB, Sartori VC, et al. Efeito fungicida dos oleos essenciais de Schinus molle L. e Schinus terebinthifolius Raddi, Anacardiaceae, do Rio Grande do Sul. Rev Bras Farmacogn. 2010;20:154-9. doi: https:// doi.org/10.1590/S0102-695X2010000200003

Lima MRF, Luna JS, Santos AF, Andrade MCC, Santana AEG, Genetj J, et al. Anti-bacterial activity of some Brazilian medicinal plants. J Ethnopharmacol. 2006;105:137-47. doi: https://doi.org/10.1016/j. jep.2005.10.026

Johann S, Cisalpino P, Watanabe GA, Cota BB, Siqueira E, Pizzolatti MG, et al. Antifungal activity of extracts of some plants used in Brazilian traditional medicine against the pathogenic fungus Paracoccidioides brasiliensis. Pharm Biol. 2010; 48(4):388-96 doi: https://doi.org/10.3109/13880200903150385

Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved Standard 3rd. ed. CLSI document M27-A3. Clinical and Laboratory Standards Institute Wayne, PA; 2008. p. 1-28.

Alves LA, Freires IA, Pereira TM, Souza A, Lima EO, Castro RD. Effect of Schinus terebinthifolius on Candida albicans growth kinetics, cell wall formation and micromorphology. Act Odontol Scand. 2013;71:965-71. doi: https://doi.org/10.3109/00016 357.2012.741694

Johann S, Silva DL, Martins CVB, Zani CL, Pizzolatti MG, Resende MA. Inhibitory effect of extracts from Brazilian medicinal plants on the adhesion of Candida albicans to buccal epithelial cells. World J Microbiol Biotechnol. 2008;24:2459-64.doi: https:// doi.org/10.1007/s11274-008-9768-5

Johann S, Sa NP, Lima LARS, Cisalpino PS, Cota BB, Alves TMA, et al. Antifungal activity of schinol and a new biphenyl compound isolated from Schinus terebinthifolius against the pathogenic fungus Paracoccidioides brasiliensis. Ann Clin Microbiol Antimicrob. 2010;9:30. doi: https://doi.org/10.1186/ 1476-0711-9-30

Degaspari CH, Waszcynskyi N, Prado MRM. Antimicrobial activity of Schinus terebenthifolius Raddi. Cienc Agrotec. 2005;29:617-22. doi: https://doi. org/10.1590/S1413-70542005000300016

Andersson H, Habel H, Olsson A, Sandhagen S, von Corswant C, Hjartstam J, et al. The influence of the molecular weight of the water-soluble polymer on phase-separated films for controlled release. Int J Pharm. 2016 Sep 10;511(1):223-35. doi: https://doi. org/10.1016/j.ijpharm.2016.06.058

Viriden A, Wittgren B, Larsson A. Investigation of critical polymer properties for polymer release and swelling of HPMC matrix tablets. Eur J Pharm Sci. 2009;36:297-309. doi: https://doi.org/10.1016/j.ejps. 2008.10.021


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